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Part 3 of 3

Supplement: five kingdoms, plant groups and viruses

After a wet autumn night, a ring of mushrooms appears on a lawn. They grow out of the ground and never move, so they look like small plants, but they are not green. A spoonful of yoghurt holds millions of living things, each one a single cell far too small to see. A drop of pond water holds single cells that swim and hunt. And the cold you caught last winter was caused by something that is not made of cells at all. Two kingdoms are not enough for all of these.

This part is Supplement content (syllabus 1.3.4–1.3.7): extra material for pupils taking the Extended papers.

A red fox, a young oak tree with its roots in the soil, and a mushroom with gills under its cap among fallen leaves. Below them, two microscope views: an amoeba with a nucleus and vacuoles, and many rod-shaped bacteria. (enlarge)

Five kingdoms

Biologists place every organism into one of five kingdoms: animals, plants, fungi, protoctists and prokaryotes. The features that decide are about the cells: how many there are, whether each has a nucleus, a cell wall and chloroplasts, and how the organism gets its food. The nucleus holds the cell's genetic material, its DNA; the cytoplasm is the jelly that fills the rest of the cell.

KingdomCellsNucleusCell wallChloroplastsFoodExamples
Animalsmanyyesnonenoneeat other living thingsfox, trout, beetle
Plantsmanyyesyes, celluloseyesphotosynthesisoak, fern, maize
Fungimost are threads called hyphae; some are single cellsyesyes, not cellulosenonedigest dead or living matter outside the bodymushroom, bread mould, yeast
Protoctistsmost are a single cellyessomesomesome like animals, some like plantsParamecium, Amoeba, Chlorella
Prokaryotessingle cellsno: a loop of DNA in the cytoplasmyesnonemany waysbacteria
The five kingdoms, one panel each with a simple drawing of their cells. Animals: many cells, no cell walls, no chloroplasts. Plants: many cells, cellulose cell walls, chloroplasts. Fungi: a branching thread with cross walls and nuclei; hyphae with walls not made of cellulose, no chloroplasts. Protoctists: one slipper-shaped cell with a nucleus, and one small green cell with a chloroplast; most are one cell, with a nucleus, some with chloroplasts. Prokaryotes: one rod-shaped cell with a wall and a loop of DNA, and no nucleus. (enlarge)

Take the three organisms that do not fit the first two kingdoms. The mushroom is only the part of a fungus that makes spores: tiny cells, each of which can grow into a new fungus. The rest is a mass of thin threads, the hyphae, spreading through the soil and the dead leaves. The hyphae have cell walls, but the walls are not made of cellulose, and there are no chloroplasts, so the fungus cannot make its own food. Instead, the hyphae release enzymes, substances that break large food molecules into small ones, onto the dead leaves. The leaves are digested outside the fungus's body, and the hyphae absorb the dissolved food. So the mushroom is a fungus. Yeast is a fungus too, even though each yeast is a single cell.

Paramecium lives in pond water. It is a single cell with a nucleus, covered in tiny hairs (cilia) that it swims with, and it takes in food like an animal. Being one cell rules out the animal and plant kingdoms, and the nucleus rules out the prokaryotes. It has no cell wall, so it is not a fungus: it is a protoctist. Other protoctists, such as Chlorella, have chloroplasts and make their own food like plants, but they are still single cells.

The bacteria in yoghurt are single cells with no nucleus: their DNA is a loop lying in the cytoplasm, and each cell has a cell wall. They are prokaryotes.

Branching thin threads with cross walls: fungal hyphae under the microscope. (enlarge)
One slipper-shaped cell covered in tiny hairs, with a large nucleus and vacuoles, and no green chloroplasts: Paramecium under the microscope. (enlarge)
Many purple rod-shaped cells: bacteria under the microscope. (enlarge)

Trap: "Mushrooms are plants." A mushroom has no chloroplasts and cannot make its own food; it digests dead material outside its body. Its hyphae have walls that are not made of cellulose. It is a fungus.

Trap: "It is one cell with a nucleus, so it must be a protoctist." Animals, plants and fungi have a nucleus in their cells too; only prokaryotes have none. And yeast is a single cell with a nucleus, yet it is a fungus: it has a cell wall that is not made of cellulose, no chloroplasts, and it absorbs its food as hyphae do. Check every feature in the table, not the nucleus alone.

Ferns and flowering plants

The plant kingdom is split into groups too. Two of them are the ferns and the flowering plants. A fern has leaves called fronds, often divided into many small leaflets, and young fronds unroll from a tight coil. A fern never has flowers or seeds. It reproduces by spores, tiny cells that can each grow into a new fern, made in tiny spore cases; on many ferns the spore cases grow in brown clusters on the underside of the fronds.

A fern with several divided fronds and a young frond still curled into a coil. One frond is turned over to show rows of brown spots on the underside of its leaflets. Roots spread into a clump of soil. (enlarge)

Trap: "The brown spots under a fern frond are a disease." They are clusters of spore cases. A fern has no flowers and no seeds; it reproduces by spores.

Flowering plants have flowers and make seeds. They are placed in two groups, named after the cotyledons, the seed leaves inside the seed. Monocotyledons (monocots), such as maize and grasses, have one cotyledon in each seed, and their leaves are usually long and narrow with parallel veins, running side by side along the leaf. Dicotyledons (dicots), such as the bean, have two cotyledons in each seed, and their leaves are usually broad, with a branching network of veins.

A fern with curled young fronds, a flowering grass with long narrow leaves, and a bean plant with pale flowers and broad leaves. Below them, a maize grain cut in half, with one seed leaf beside the food store, and a bean seed split into its two large halves, with the young shoot between them. (enlarge)

Maize and bean show the difference. Soak a maize grain and cut it lengthwise: most of it is a food store, with one small cotyledon on one side. Soak a bean and it splits into two large halves: the two cotyledons, with the young shoot and root between them. The leaves agree. A maize leaf is long and narrow, with veins running side by side from base to tip. A bean leaf is broad, and its veins branch again and again into a net. So maize is a monocot and the bean is a dicot.

Monocotyledons and dicotyledons compared. Monocotyledons, such as maize, grasses and lilies: a maize grain cut lengthwise with a large food store and one cotyledon; a long, narrow leaf with parallel veins. Dicotyledons, such as bean, oak and daisy: a bean seed opened into two cotyledons; a broad leaf with a branching network of veins. (enlarge)

Trap: "Grass has no flowers, so it is not a flowering plant." Grass flowers are small and green, with no bright petals, but they are flowers, and they make seeds: wheat, rice and maize grains are seeds. Grasses are monocots.

Viruses

A virus is not made of cells. It is a tiny particle: genetic material (DNA or RNA) inside a protein coat. It has no cytoplasm and no ribosomes (the tiny parts of a cell that make proteins), it does not feed, respire or grow, and it is far smaller than a bacterium: you need an electron microscope to see one. A virus can make copies of itself only inside the living cells of another organism. Because it is not made of cells, a virus is not placed in any of the five kingdoms.

A virus. A six-sided protein coat made of many small protein units, with a tangled strand of genetic material, DNA or RNA, inside it. Notes: not made of cells; far smaller than a bacterium. (enlarge)

Trap: "Viruses are bacteria." A bacterium is a living cell, with a cell wall, a membrane, cytoplasm and a loop of DNA: it is a prokaryote. A virus is not a cell at all, only genetic material inside a protein coat.

The last stage of the experiment brings these organisms to the bench: the mushroom, Paramecium, the yoghurt bacteria, the fern, maize, the bean and a virus. Make a first guess for each if you like; then look closer, through the hand lens or at a microscope print, before you choose its kingdom or plant group. The virus has its own dish: not in any kingdom.

•••
PondweedPlantsPlant kingdomFernsMonocotsDicotsFungiProtoctistsProkaryotesFunguskingdomProtoctistkingdomProkaryotekingdomNot in anykingdomAnimal kingdomVertebratesArthropodsTroutFishNewtAmphibiansLizardReptilesPenguinBirdsBatMammalsGround beetleInsectsGarden spiderArachnidsWoodlouseCrustaceansCentipedeMyriapodsSea anemoneOther animalsOrganismcards6 leftMicroscopeprintsMushroom

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Key idea: (Supplement) Every organism is placed in one of five kingdoms: animals, plants, fungi (hyphae with walls not made of cellulose, no chloroplasts, digest food outside the body), protoctists (mostly single cells with a nucleus) and prokaryotes (single cells with no nucleus and a cell wall). Ferns have fronds and make spores, not flowers or seeds. Flowering plants are monocotyledons (one cotyledon, parallel veins) or dicotyledons (two cotyledons, branching network of veins). A virus is genetic material inside a protein coat; it is not made of cells and is in no kingdom.

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Scope and sources

Cambridge IGCSE Biology 0610 · 2026–2028 v3 · Original learning materials.

The main features used to place animals and plants into their kingdoms (1.3.1), the main features of the five vertebrate groups and the four arthropod groups (1.3.2) and classifying organisms with them (1.3.3) in the reading, the practice and the sorting experiment; (Supplement) the main features of the five kingdoms (1.3.4), of ferns and of flowering plants, monocotyledons and dicotyledons (1.3.5), classifying with them (1.3.6) and the features of viruses, a protein coat and genetic material (1.3.7), in part 3 and the Supplement cards.

Apparatus, amounts and times in the experiment are illustrative. Real practical work follows a teacher-approved safety method.

Official Cambridge syllabus